Hdl Direct Measure Peg
The combination of peg modified enzymes with alpha cyclodextrin sulfate provided selectivity for the determination of.
Hdl direct measure peg. Direct method for example measures hdl cholesterol di rectly in unprocessed serum samples by using polyethylene glycol peg modified cholesterol esterase and cholesterol. 09 14 hdl direct cholesterol page 2 4 reagent 2. Well accepted now is the inverse association between hdl cholesterol hdl c 1 and risk of developing coronary artery disease cad a relationship that persists after adjustment for other risk factors and is consistent with putative protective mechanisms the measurement of hdl c in characterizing risk for cad and managing treatment of dyslipidemia has become increasingly common in. Plasma or serum hdl c concentration is commonly determined by precipitation methods using various reagents.
A high density lipoprotein hdl test measures the level of good cholesterol in your blood. A protocol of the direct method for example measures hdl cholesterol directly in unprocessed serum samples by using polyethylene glycol peg modified cholesterol esterase and cholesterol. The test for high density lipoprotein cholesterol hdl c is used as part of a lipid panel to screen for unhealthy levels of lipids and to determine your risk of developing heart disease and help make decisions about what treatment to use if you have borderline risk intermediate risk or high risk. Peg precipitation preserves the integrity of pre β hdl particles.
When cholesterol esterase and cholesterol oxidase enzymes were modified with peg they s. Cholesterol is a waxy substance that s found in all of the cells in your body. Plasma high density lipoprotein cholesterol. Hdl cholesterol esters are converted to hdl cholesterol by peg cholesterol esterase.
Direct measurement of high density lipoprotein cholesterol in serum with polyethylene. H okabe t irie k uekama n kayahara k miyauchi direct measurement of high density lipoprotein cholesterol in serum with. The other components of a lipid panel typically include total cholesterol ldl cholesterol ldl. We have developed an automated method for measuring high density lipoprotein hdl cholesterol in serum without prior separation using polyethylene glycol peg modified enzymes and sulfated alpha cyclodextrin.
The hdl cholesterol is acted upon by peg cholesterol oxidase. Calibrations are defined for the direct hdl c assay.